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With the development of synthetic biotechnology, in vitro biosynthesis has gradually become one of the important ways of chemical synthesis, with the advantages of environmental friendliness, high catalytic efficiency, good atomic economy and strong controllability. The use of biological macromolecules to assemble enzyme elements into multi-enzyme complexes can improve the reaction rate of biosynthesis in vitro and reduce the occurrence of side reactions. Three kinds of common biological macromolecules used for the assembly of enzyme elements, including connective peptides, protein scaffolds, DNA, etc. In recent years, the application of in vitro biosynthesis in the production of bulk chemicals (sugar chemicals, organic acid chemicals, alcohol chemicals, etc.) shows the application prospect of in vitro biosynthesis in chemical synthesis. As an important means of in vitro biosynthesis, mRNA display technology can be used to design random DNA sequences and achieve high-throughput in vitro screening of substrates to develop a variety of naturally difficult to produce small molecular compounds with special structures. It can be applied to various fields of chemical industry production and realize in vitro biosynthesis of chemicals.
Compared with the traditional methods used in the production of chemicals, the use of in vitro synthesis technology has the following advantages: improve the production process, increase the yield, potency and quality; It can produce new products with biodegradability, low toxicity and excellent characteristics; Save time and money by increasing renewable and cheaper sources of raw materials and simplifying and optimizing production processes. CD BioSciences is committed to using mRNA display technology to achieve the synthesis and development of target molecules, and integrate them into chemical in vitro biosynthesis, for example, to develop biological macromolecules for multi-enzyme assembly, including linker peptides, protein scaffolds and DNA.
Glucosamine is widely used in the food, cosmetic and pharmaceutical industries. The methods currently used to produce glucosamine mainly include chemical methods and microbial fermentation methods. The chemical method requires strong acid hydrolysis and complex operation steps, and is accompanied by the generation of toxic by-products. The glucosamine produced by microbial fermentation is easily consumed by microbial metabolism, and the accumulation of products will inhibit the growth of microorganisms and reduce the yield. Using in vitro display technology to develop an efficient enzyme-catalyzed system for the biosynthesis of sugar chemicals can increase the yield and molar conversion rate of sugar, and has the advantages of good atom economy and strong controllability, and has certain industrial application prospects.
α-Ketoglutarate is an important intermediate in the tricarboxylic acid cycle and amino acid synthesis, and can be used as a dietary supplement, a raw material for wound healing compounds, and a basic raw material for heterocyclic chemical synthesis. At present, α-ketoglutarate is generally chemically synthesized using succinic acid and diethyl oxalate with cyanohydrin, and the yield is about 75%, but due to the use of toxic raw materials, this method will produce toxic wastes (such as cyanide ). Environmentally friendly biosynthesis can be used as an alternative to chemical methods, but in vivo biosynthesis requires precise control of its culture conditions (such as thiamine and nitrogen concentrations, pH and aeration, etc.), and the side effects such as pyruvic acid and other organic acids are produced. Product inhibits cell growth. Therefore, designing an uronic acid-like oxidation pathway and using mRNA display technology to construct an enzyme system for screening high catalytic efficiency and stability can achieve large-scale industrial production.
Ethanol is a commonly used chemical, which is widely used in chemical industry, medical treatment, food and agricultural production. In the microbial fermentation production of ethanol, the low tolerance of bacteria to temperature and solvent conditions is the main reason for the low conversion efficiency. An in vitro biosynthesis system that synthesizes ethanol from glucose can be designed and constructed to achieve green and high-conversion production of ethanol. The system uses a variety of enzymes as catalysts to construct a high-efficiency intermediate platform for the synthesis of pyruvate from glucose, and then uses a complex enzyme system to catalyze the synthesis of ethanol from pyruvate. The mRNA display technology can screen and optimize multiple alcohol complex enzyme systems to prepare more efficient catalytic systems.
CD BioSciences is committed to discovering a variety of functional compounds through mRNA display technology, which can be used in the green production and transformation of various chemicals in the chemical industry. If you have any applications or services you want to know about, please contact us now to get the best advice and solutions. If you have any applications or services you want to know, please contact us immediately for the best suggestions and solutions.
