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Compared with traditional hybridoma technology, mRNA display technology has outstanding advantages in cycle time, screening scope, cost and expense. The process of mRNA display screening library is simple and efficient, which can obtain antibodies with strong affinity in a short time. CD BioSciences has devoted itself to the research of antibody field for many years, accumulated rich experience in antibody discovery, and can provide customers with multi type antibody discovery services based on mRNA display technology.
mRNA display technology is to use genetic engineering technology to synthesize or extract antibody genes and transcribe them into mRNA in vitro, connect the mRNA with the antibody obtained in the process of in vitro translation through puromycin, and then reverse transcribe to form a ternary complex and combine it with the target protein, complete the screening of antibody under mRNA display, and obtain high affinity and high specificity target antibody through multiple rounds of elutriation. Based on mRNA display technology, it can realize the discovery of many kinds of antibodies, including mouse monoclonal antibody, rabbit monoclonal antibody, chicken monoclonal antibody and all human monoclonal antibody.
Fig. 1 mRNA display cycle. (Kamalinia, et al., 2021)
mRNA Display has several significant advantages over other display techniques as a protein design tool：(1) higher diversity; (2) lack of context dependence; (3) strictly monomeric libraries; (4) the ability to use an expanded genetic code; (5) ease of use with high throughput sequence analysis. Compared with other methods such as phage display, mRNA display technology improves the diversity of the library by 104 times, so it is a better antibody discovery method.
mRNA display technology includes sequence design and synthesis, antibody library construction, elution, identification and other steps. CD BioSciences uses flexible sequence design strategies, multiple screening methods (antigens, cells), and affinity and specificity testing and verification to provide comprehensive and efficient antibody discovery services.
Antibodies from humans, mice, rabbits, sheep, camels and other species were found to be compatible.
From sample collection to antibody library screening, the experimental cycle was significantly shortened.
Using powerful in vitro mRNA display technology, the capacity of antibody library can reach 1014.
Multiple mutations can be introduced, not limited to cell transformation and other processes.
CD BioSciences has many years of project development experience in antibody discovery. Based on the mRNA display technology platform, it can provide customers with antibody discovery services such as antibody library construction, antibody screening and antibody affinity maturation. If you are interested in our service, please contact us for more details.
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