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The new generation of self-assembly protein chip can express and fix proteins in real time and in situ on the chip by using cell-free expression system and DNA fixation technology, effectively solving the problems of preparation and preservation of traditional protein chips, and has been initially used in large-scale protein-protein interaction screening and identification of immune dominant antigens. CD BioSciences is committed to applying in vitro display technology to the field of protein research, and has accumulated rich experience in protein engineering for many years. It can provide customers with more flexible, fast and cost-effective development services for self-assembled protein microarrays.
Two basic features are required for function-based protein molecular screening: first, functional information (natural structure of protein) is obtained through high-throughput analysis of rich protein libraries; Secondly, the genetic information (DNA sequence) is decoded from the analyzed proteins by linking the expressed phenotype with its coding genotype. Protein microarray provides a means for high-throughput analysis and molecular display technology, in which genotype and phenotype are physically linked, providing sequence information for identification purposes. However, in order to be truly effective, future complex proteomics research will need to carry out molecular screening for very large populations (super protein libraries) in a side-by-side manner and under the conditions of required functions.
Fig. 1 Application of Functional Protein Microarray. (Syu G D, et al., 2020)
The integration of molecular display technology, such as mRNA display, is known as the most simplified molecular display and a powerful means of screening the most diverse mutant library. It will provide the revolutionary improvements needed to significantly improve the screening ability of biological molecules and ultra-high throughput and function-based screening on the microarray platform. It provides powerful technical support for obtaining important life information (such as unknown protein components, sequences, biological functions at the level of expression in vivo, regulatory relationships with other molecules, drug screening, drug target selection, etc.).
Protein self-assembly microarray is a high-throughput protein function analysis technology, which can be used to analyze protein expression profile, study protein-protein interaction, even DNA-protein, RNA-protein interaction, and screen protein targets for drug action. The protein discovery platform built by CD BioSciences based on mRNA display technology can provide one-stop service for the development of protein self-assembly microarray for global customers.
Our services are applicable to a variety of research and analysis purposes (protein interaction analysis, drug target selection, etc.). We can develop a variety of self-assembly protein chips such as enzymes, antigens, antibodies, receptors, ligands, etc., and capture the proteins to be tested that can specifically bind to them (exist in serum, plasma, lymph, interstitial fluid, urine, exudates, cell lysates, and secretions) according to the characteristics of these biological molecules.
CD BioSciences has many years of project development experience in protein discovery. The protein discovery platform based on mRNA display technology can provide customers with the development services of a variety of self-assembled protein microarrays. If you are interested in our services, please contact us for more details.
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