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The antibody genes of the immune antibody library are derived from the lymphocytes and tissues of animals or humans immunized with antigens. Such antibody libraries contain antigen specific antibodies with high abundance and are helpful to screen antibodies with high affinity. CD BioSciences can provide customers with the design and construction of immune antibody libraries based on its mRNA display technology.
The immune antibody library is constructed from B cells of immune or infected individuals targeting specific antigens of interest. After exposure to antigen, in vivo affinity maturation and V gene hypermutation contribute to the formation of a specific gene pool for antigen. Therefore, antibodies isolated from immune libraries usually exhibit higher affinity in the nanomolar range. Like naïve antibody libraries, antibody genes can also be isolated from PBMC, bone marrow, lymph nodes or spleen. IgG is usually used to build immune antibody libraries, while IgA and IgE are sometimes used for libraries based on mucosa, parasites and allergies. Immune antibody libraries are usually customized for a specific set of antigens associated with a specific infection. Although the separation of binders for closely related antigens has been reported, the immune library is usually not used for different antigens, because it is generally believed that the skewed gene library will mean the key application of the immune library.
mRNA display can isolate high affinity monoclonal antibodies from libraries constructed by immunized animals. The typical size of these libraries is between 1012 - 1014, reflecting the immune response of animals. High affinity antibodies can be isolated directly from them by mRNA display technology. CD BioSciences have rich experience in building antibody libraries of different formats and types. You only need to provide antigens or our technical platform can design and synthesize antigens for you, and then we can carry out immune activities to design and build immune antibody libraries for you.
Construction Process of mRNA Display Immune Antibody Library
CD BioSciences has many years of project development experience in antibody discovery. Based on the mRNA display technology platform, it can provide customers with various types of antibody library construction services. If you are interested in our services, please contact us for more details.
