Affinity Selection

Affinity selection is a critical step in mRNA display, essential for screening desired protein variants. CD BioSciences harnesses the power of mRNA display technology to advance peptide drug discovery. Our cutting-edge approach leverages the unique capabilities of mRNA display to identify high-affinity peptides rapidly and efficiently.

Fig 1. General scheme for peptide selection by mRNA display.

Affinity Selection in mRNA Display

In the early 90s, the researcher described the use of new technology for linking genotype and phenotype to select for new affinity molecules in vast libraries using in vitro selection principles. Affinity selection is fundamental to many fields, including pharmacology, biotechnology, and molecular biology, due to its ability to isolate molecules with specific and high-affinity interactions. Identifying peptides that bind to therapeutic targets with high specificity and affinity, which can lead to the development of novel drugs.

Fig 2. In vitro selection of peptides and proteins. (Huang, et al., 2018)

Our Services

After reverse transcription, CD BioSciences binds the target protein to magnetic beads and incubates them with our constructed library to screen for mRNA/cDNA-protein fusions that bind the target. When a biomolecule, such as a protein, is bound, an external magnetic field causes the beads to adhere to the outer edge of the container. During washing, the immobilized beads retain the bead-bound DNA.

Create a diverse library of mRNA/cDNA-peptide fusions. Library Construction Remove weak binders and elute those with strong affinity. Washing and Elution

Incubation with Target Mix the library with the target to allow binding of high-affinity peptides. Reverse Transcription and Amplification Convert selected mRNAs to cDNA, amplify them, and use them to generate more mRNA-peptide fusions for further rounds of selection.

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CD BioSciences' expertise in mRNA display positions us at the forefront of peptide drug discovery. Our comprehensive services are designed to accelerate your research and development efforts, providing high-quality leads and actionable insights. Partner with us to fully leverage the potential of mRNA display in your drug discovery endeavors. Contact us for more information.

Reference

1. Huang, Y., et al. RNA display methods for the discovery of bioactive macrocycles. Chemical Reviews. 2018, 119(17): 10360-10391.

Related Services

• In Vitro Transcription
• In Vitro Translation
• Analysis and Optimization
• DNA Library Preparation
• Peptide Cyclization
• Puromycin Ligation
• Reverse Transcription